1QX1

Golgi alpha-mannosidase II D341N mutant complex with 2-F-mannosyl-F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-03-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	69.046, 109.826, 138.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.300
R-factor	0.17
Rwork	0.170
R-free	0.18800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hty
RMSD bond length	0.020 *
RMSD bond angle	2.020 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.380
High resolution limit [Å]	1.300	1.300
Rmerge	0.106	0.586
Total number of observations	1589410 *
Number of reflections	246051
<I/σ(I)>	14.7	2.9
Completeness [%]	95.3	89.7 *
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	298	van den Elsen, J.M., (2001) EMBO J., 20, 3008. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	8.5 (%)
2	1	reservoir	MPD	5 (%)
3	1	reservoir	Tris	100 (mM)	pH7.0