1QWR
Crystal Structure Analysis of the Mannose 6-Phosphate Isomerase from Bacillus subtilis
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-03 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97932, 0.97953, 0.956 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.116, 72.534, 70.309 |
| Unit cell angles | 90.00, 91.89, 90.00 |
Refinement procedure
| Resolution | 45.810 - 1.800 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.23200 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.074 | 0.312 |
| Number of reflections | 54846 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.2 | 94.4 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Litium sulfate, PEG 3350, BisTris, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
