1QWK

Structural genomics of Caenorhabditis Elegans: Hypothetical 35.2 kDa protein (aldose reductase family member)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2003-07-21
Detector	MARRESEARCH
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	65.050, 89.570, 116.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 1.600
R-factor	0.201
Rwork	0.200
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1az2
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.075	0.243
Number of reflections	41312
<I/σ(I)>	12.3	3.8
Completeness [%]	91.7	64.1
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1