1QW2
Crystal Structure of a Protein of Unknown Function TA1206 from Thermoplasma acidophilum
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-03-06 |
Detector | CUSTOM-MADE |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 35.642, 48.060, 110.382 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.220 - 1.500 |
R-factor | 0.203 |
Rwork | 0.203 |
R-free | 0.21800 |
Structure solution method | MAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.069 | 0.248 |
Number of reflections | 24601 | |
<I/σ(I)> | 35.8 | 4 |
Completeness [%] | 83.7 | 52.9 |
Redundancy | 7.4 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 0.2M Magnesium Nitrate, 30% PEG 3350, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |