1QVZ

Crystal structure of the S. cerevisiae YDR533c protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM30A
Synchrotron site	ESRF
Beamline	BM30A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-03-12
Detector	MARRESEARCH
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	61.404, 165.927, 47.807
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.850
R-factor	0.191
Rwork	0.191
R-free	0.21500
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	23.100 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.730	1.730
Rmerge	0.040 *	0.180 *
Total number of observations	336312 *
Number of reflections	95497
<I/σ(I)>	18	3.8
Completeness [%]	96.4	78.9
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.4	293	25-30% PEG 4000, 50mM Na/K phosphate buffer, 20mM DTT, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	reservoir	PEG4000	25-30 (%)
3	1	reservoir	sodium potassium phosphate	50 (mM)	pH6.4
4	1	reservoir	dithiothreitol	20 (mM)