1QVW

Crystal structure of the S. cerevisiae YDR533c protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-11-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 2
Unit cell lengths	61.531, 166.196, 48.295
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 1.900
Rwork	0.185
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Structure of YDR533c solved by SAD method
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.960
High resolution limit [Å]	1.900	1.900
Rmerge	0.081 *	0.440 *
Total number of observations	185333 *
Number of reflections	38837
<I/σ(I)>	21.4	5
Completeness [%]	97.8	93.7
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.4	293	25-30% PEG 4000, 50mM Na/K phosphate buffer, 20mM DTT, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	24 (mg/ml)
2	1	reservoir	PEG4000	25-30 (%)
3	1	reservoir	sodium potassium phosphate	50 (mM)	pH6.4
4	1	reservoir	dithiothreitol	20 (mM)