1QVR
Crystal Structure Analysis of ClpB
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-03-16 |
| Detector | SBC-2 |
| Wavelength(s) | 0.9793, 0.9795, 1.0197 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 107.747, 138.482, 212.215 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.000 * - 3.000 |
| R-factor | 0.263 |
| Rwork | 0.263 |
| R-free | 0.30700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.130 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 * | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.053 * | 0.381 * |
| Number of reflections | 107224 | |
| <I/σ(I)> | 19.1 | |
| Completeness [%] | 91.4 | 37.1 |
| Redundancy | 2.4 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 291 | PEG 4000, sodium acetate, potassium chloride, glycerol, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 291 | PEG 4000, sodium acetate, potassium chloride, glycerol, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 291 | PEG 4000, sodium acetate, potassium chloride, glycerol, pH 4.1, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 10 | 1 | reservoir | glycerol | 20 (%) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | pH7.6 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | glycerol | 5 (%) | |
| 6 | 1 | drop | AMPPNP | 5 (mM) | |
| 7 | 1 | reservoir | PEG4000 | 4 (%(w/v)) | |
| 8 | 1 | reservoir | sodium acetate | 200 (mM) | pH4.1 |
| 9 | 1 | reservoir | 200 (mM) |
