1QVG
Structure of CCA oligonucleotide bound to the tRNA binding sites of the large ribosomal subunit of Haloarcula marismortui
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Detector technology | CCD |
| Collection date | 2003-04-09 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.9999 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 213.517, 300.612, 573.929 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| Rwork | 0.201 |
| R-free | 0.26200 * |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1jj2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.900 * | 2.900 * |
| Total number of observations | 2687658 * | |
| Number of reflections | 443616 | |
| <I/σ(I)> | 17.6 | 2 |
| Completeness [%] | 99.9 * | 100 * |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | back-extraction, vapor diffusion * | 7.1 * | Ban, N., (2000) Science, 289, 905. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.2 (M) | ||
| 2 | 1 | reservoir | 0.5 (M) | ||
| 3 | 1 | reservoir | Kacetate | 100 (mM) | |
| 4 | 1 | reservoir | 30 (mM) | ||
| 5 | 1 | reservoir | PEG6000 | 7 (%) | |
| 6 | 1 | reservoir | Tris | 15 (mM) | |
| 7 | 1 | reservoir | MES | 15 (mM) | |
| 8 | 1 | reservoir | 1 (mM) |
