1QV9
Coenzyme F420-dependent methylenetetrahydromethanopterin dehydrogenase (Mtd) from Methanopyrus kandleri: A methanogenic enzyme with an unusual quarternary structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-11 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 120.100, 151.200, 110.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.920 - 1.540 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.22700 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.620 |
| High resolution limit [Å] | 1.600 * | 1.550 |
| Rmerge | 0.075 | 0.257 |
| Number of reflections | 145808 | |
| <I/σ(I)> | 17.7 | 3.9 |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | Mops/KOH, MPD, Mg acetate, Na phosphate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12 (mg/ml) | |
| 2 | 1 | drop | MOPS-KCH | 10 (mM) | pH7.0 |
| 3 | 1 | reservoir | MPD | 13 (%(v/v)) | |
| 4 | 1 | reservoir | sodium phosphate | 0.1 (M) | pH8.0 |
| 5 | 1 | reservoir | magnesium acetate | 0.2 (M) |
