1QUS

1.7 A RESOLUTION STRUCTURE OF THE SOLUBLE LYTIC TRANSGLYCOSYLASE SLT35 FROM ESCHERICHIA COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	120
Detector technology	CCD
Collection date	1995-04-09
Detector	PRINCETON 2K
Spacegroup name	P 21 21 21
Unit cell lengths	58.329, 67.883, 98.853
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.178
Rwork	0.176
R-free	0.20700
RMSD bond length	0.008
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.050	0.261
Total number of observations	206582 *
Number of reflections	43117
<I/σ(I)>	24.4
Completeness [%]	97.6	91.8
Redundancy	4.8	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.8	295	BICINE-NAOH, PEG 20K, ISOPROPANOL, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Bicine-NaOH	0.1 (M)
2	1	1	PEG20000	0-6 (%)