1QUO
L99A/E108V MUTANT OF T4 LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.970, 60.970, 97.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.172 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.600 |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.050 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.223 |
| Number of reflections | 15757 | |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 89.0 | 82 |
| Redundancy | 2.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | phosphate | 2.0 (M) | |
| 3 | 1 | reservoir | 0.25 (M) | ||
| 4 | 1 | reservoir | oxidized beta-mercaptoethanol |
