1QUL
PHOSPHATE-BINDING PROTEIN MUTANT WITH ASP 137 REPLACED BY THR COMPLEX WITH CHLORINE AND PHOSPHATE
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1995-01-09 |
| Detector | SDMS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.970, 63.980, 123.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.700 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.20500 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.720 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Rmerge | 0.065 |
| Number of reflections | 37024 |
| Completeness [%] | 83.0 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 * | Wang, Z., (1994) J.Biol.Chem., 269, 25091. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.25 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 1.5 (mM) | |
| 3 | 1 | drop | PEG6000 | 13.5 (%(w/v)) | |
| 4 | 1 | drop | 37.5 (mM) | ||
| 5 | 1 | drop | potassium acetate | 15 (mM) | |
| 6 | 1 | reservoir | PEG6000 | 18 (%(w/v)) | |
| 7 | 1 | reservoir | 50 (mM) | ||
| 8 | 1 | reservoir | potassium phosphate | 2 (mM) | |
| 9 | 1 | reservoir | potassium acetate | 20 (mM) |
