1QU9
1.2 A CRYSTAL STRUCTURE OF YJGF GENE PRODUCT FROM E. COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 75 |
| Detector technology | CCD |
| Collection date | 1998-05-31 |
| Detector | BRUKER |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 72.220, 72.220, 72.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.200 |
| Rwork | 0.164 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.031 |
| Data reduction software | SMART |
| Data scaling software | SMART |
| Phasing software | MLPHARE |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 112350 | |
| Completeness [%] | 94.8 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.1 | 277 | 50 mM tris HCL, 2.35 M ammonium sulphate, pH 8.1, microdialysis, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 50 (mM) | |
| 2 | 1 | 1 | ammonium sulfate | 2.35 (M) |
