1QTV

T26E APO STRUCTURE OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	75
Detector technology	AREA DETECTOR
Collection date	1999-03-29
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	61.220, 61.220, 96.320
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.000 - 2.300
R-factor	0.184
RMSD bond length	0.016
RMSD bond angle	2.600
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.480
High resolution limit [Å]	2.300	2.300
Rmerge	0.070	0.223
Total number of observations	30082 *
Number of reflections	9358
<I/σ(I)>	7.2
Completeness [%]		92
Redundancy	3.2	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *			Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	2.0 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	beta-mercaptoethanol