1QTQ
GLUTAMINYL-TRNA SYNTHETASE COMPLEXED WITH TRNA AND AN AMINO ACID ANALOG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 238.780, 93.360, 115.260 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.250 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.25400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gtr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 27.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 2.300 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.084 | 0.534 |
| Number of reflections | 51376 | |
| Completeness [%] | 88.8 | 67.7 |
| Redundancy | 2.5 | 1.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 17 * | 2.0 M AMMONIUM SULFATE, 20 MM MGSO4, 80 MM PIPES, PH 7.0, 1 MM QSI, AND 1:1 MOLAR RATIO OF TRNA TO PROTEIN AT 17 DEGREES C BY THE HANGING DROP METHOD., vapor diffusion - hanging drop, temperature 290.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | AMMONIUM SULFATE | ||
| 10 | 1 | 3 | PIPES | ||
| 11 | 1 | 3 | SODIUM AZIDE | ||
| 2 | 1 | 1 | MGSO4 | ||
| 3 | 1 | 1 | PIPES | ||
| 4 | 1 | 2 | AMMONIUM SULFATE | ||
| 5 | 1 | 2 | MGSO4 | ||
| 6 | 1 | 2 | PIPES | ||
| 7 | 1 | 3 | AMMONIUM SULFATE | ||
| 8 | 1 | 3 | MGSO4 | ||
| 9 | 1 | 3 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | tRNA enzyme | ||
| 2 | 1 | reservoir | PIPES | 80 (mM) | |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | reservoir | beta-mercaptoethanol | 20 (mM) | |
| 5 | 1 | reservoir | QSI | 1 (mM) |
