1QTO

1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6B
Synchrotron site	Photon Factory
Beamline	BL-6B
Temperature [K]	298
Detector technology	DIFFRACTOMETER
Collection date	1996-05-08
Detector	WEISSENBERG
Spacegroup name	P 21 21 2
Unit cell lengths	54.940, 67.880, 35.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.500
Rwork	0.190
R-free	0.22100
Structure solution method	SIRAS
RMSD bond length	0.005
RMSD bond angle	26.900 *
Data reduction software	WEIS
Data scaling software	CCP4 ((AGROVATA)
Phasing software	DM
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.420	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.054	0.139
Total number of observations	74090 *
Number of reflections	19723 *
<I/σ(I)>	7.95
Completeness [%]	94.3	85.7
Redundancy	3.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	20 *	drop consists of equal volume of protein and reservoir solutions *
1	VAPOR DIFFUSION, HANGING DROP	5.7	20 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	PEG4000	30 (%)