1QTO
1.5 A CRYSTAL STRUCTURE OF A BLEOMYCIN RESISTANCE DETERMINANT FROM BLEOMYCIN-PRODUCING STREPTOMYCES VERTICILLUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 298 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-05-08 |
| Detector | WEISSENBERG |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 54.940, 67.880, 35.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| Rwork | 0.190 |
| R-free | 0.22100 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 26.900 * |
| Data reduction software | WEIS |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | DM |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.420 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.054 | 0.139 |
| Total number of observations | 74090 * | |
| Number of reflections | 19723 * | |
| <I/σ(I)> | 7.95 | |
| Completeness [%] | 94.3 | 85.7 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 20 * | drop consists of equal volume of protein and reservoir solutions * |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 20 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | PEG4000 | 30 (%) |
