1QTJ
CRYSTAL STRUCTURE OF LIMULUS POLYPHEMUS SAP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 298 |
| Detector technology | FILM |
| Collection date | 1989-01-01 |
| Detector | CEA |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 173.330, 173.330, 98.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| Rwork | 0.397 |
| R-free | 0.40100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HUMAN C-REACTIVE PROTEIN PROTOMER (1GNH) |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 3.130 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.117 | 0.284 |
| Total number of observations | 47412 * | |
| Number of reflections | 13666 | |
| Completeness [%] | 89.0 | 55.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 8 (%) | |
| 2 | 1 | reservoir | 10 (mM) | ||
| 3 | 1 | reservoir | MES |
