1QTE

CRYSTAL STRUCTURE OF THE 70 KDA SOLUBLE LYTIC TRANSGLYCOSYLASE SLT70 FROM ESCHERICHIA COLI AT 1.90 A RESOLUTION IN COMPLEX WITH A 1,6-ANHYDROMUROTRIPEPTIDE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	120
Detector technology	CCD
Collection date	1995-04-09
Detector	PRINCETON 2K
Spacegroup name	P 21 21 21
Unit cell lengths	78.266, 87.514, 132.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.172 *
Rwork	0.171
R-free	0.19700
RMSD bond length	0.007
RMSD bond angle	19.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.930
High resolution limit [Å]	1.900	1.900
Rmerge	0.059	0.148
Total number of observations	193873 *
Number of reflections	57525
<I/σ(I)>	17.9
Completeness [%]	79.3	63.7 *
Redundancy	3.4	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.8 *	295	Rozeboom, H.J., (1990) J. Mol. Biol., 212, 557. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	15-30 (%)
2	1	reservoir	sodium acetate	0.1 (M)	pH4.5-5.0
3	1	drop	protein	10 (mg/ml)
4	1	drop	potassium phosphate	10 (mM)	pH6.8