1QTE
CRYSTAL STRUCTURE OF THE 70 KDA SOLUBLE LYTIC TRANSGLYCOSYLASE SLT70 FROM ESCHERICHIA COLI AT 1.90 A RESOLUTION IN COMPLEX WITH A 1,6-ANHYDROMUROTRIPEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 1995-04-09 |
| Detector | PRINCETON 2K |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.266, 87.514, 132.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.172 * |
| Rwork | 0.171 |
| R-free | 0.19700 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 19.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.059 | 0.148 |
| Total number of observations | 193873 * | |
| Number of reflections | 57525 | |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 79.3 | 63.7 * |
| Redundancy | 3.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 * | 295 | Rozeboom, H.J., (1990) J. Mol. Biol., 212, 557. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 15-30 (%) | |
| 2 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.5-5.0 |
| 3 | 1 | drop | protein | 10 (mg/ml) | |
| 4 | 1 | drop | potassium phosphate | 10 (mM) | pH6.8 |
