1QT4

T26Q MUTANT OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Detector technology	AREA DETECTOR
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 32 2 1
Unit cell lengths	61.090, 61.090, 96.288
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
R-factor	0.163
RMSD bond length	0.015
RMSD bond angle	2.800
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.020
High resolution limit [Å]	1.870	1.870
Rmerge	0.043 *	0.253
Total number of observations	33225 *
Number of reflections	15148
<I/σ(I)>	9.8
Completeness [%]	81.0	55
Redundancy	2.2	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7 *		Eriksson, A.E., (1993) J. Mol. Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	2.0 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	beta-mercaptoethanol