1QSW

CRYSTAL STRUCTURE ANALYSIS OF A HUMAN LYSOZYME MUTANT W64C C65A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1991-02-12
Detector	MACSCIENCE DIP100S
Spacegroup name	P 1
Unit cell lengths	45.920, 107.240, 32.080
Unit cell angles	98.59, 110.45, 78.04

Refinement procedure

Resolution	10.000 - 1.850
R-factor	0.181 *
Rwork	0.181
R-free	0.26100
RMSD bond length	0.013
RMSD bond angle	0.039
Data reduction software	PROTEIN
Data scaling software	PROTEIN
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.940
High resolution limit [Å]	1.850	1.850
Rmerge	0.066	0.163 *
Total number of observations	76633 *
Number of reflections	43715	3092 *
Completeness [%]	80.4	64.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	298	SODIUM PHOSPHATE, NACL, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 25K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	2	sodium phosphate	30 (mM)
3	1	2		2.5 (M)