1QSQ

CAVITY CREATING MUTATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	FILM
Collection date	1991-07-25
Detector	KODAK
Spacegroup name	P 32 2 1
Unit cell lengths	60.900, 60.900, 96.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.153
Rwork	0.153
RMSD bond length	0.018
RMSD bond angle	2.265
Data scaling software	CCP4 ((AGROVATA)
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	1.900
High resolution limit [Å]	1.850	1.850
Rmerge	0.046	0.258
Number of reflections	11951
<I/σ(I)>	10
Completeness [%]	64.0	67
Redundancy	2.2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.7 *	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *
1	Vapor diffusion *	6.7 *	277	Eriksson, A.E., (1993) J.Mol.Biol., 229, 747. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir		0.25 (M)
3	1	reservoir	phosphate	2.0 (M)
4	1	reservoir	beta-mercaptoethanol