1QSN
CRYSTAL STRUCTURE OF TETRAHYMENA GCN5 WITH BOUND COENZYME A AND HISTONE H3 PEPTIDE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Detector technology | CCD |
Collection date | 1998-09-11 |
Detector | CUSTOM-MADE |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 65.090, 65.090, 96.630 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.239 |
Rwork | 0.239 |
R-free | 0.26600 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.340 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.210 |
High resolution limit [Å] | 2.000 | 2.150 |
Rmerge | 0.047 | 0.066 |
Total number of observations | 201683 * | |
Number of reflections | 12960 | |
<I/σ(I)> | 33.3 | |
Completeness [%] | 96.9 | 77.9 |
Redundancy | 15.1 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 25 * | TRIS, AMMONIUM SULFATE, MANGANESE CHLORIDE, pH 7.50, VAPOR DIFFUSION, HANGING DROP, temperature 298.00K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7.5 (mg/ml) | |
2 | 1 | drop | CoA | 1 (mM) | |
3 | 1 | drop | peptide | 1.5 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 1.6 (M) | |
5 | 1 | reservoir | Tris | 50 (mM) | |
6 | 1 | reservoir | 10 (mM) |