1QSL

KLENOW FRAGMENT COMPLEXED WITH SINGLE-STRANDED SUBSTRATE AND EUROPIUM (III) ION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1997-05-17
Detector	ADSC
Spacegroup name	P 43
Unit cell lengths	103.300, 103.300, 86.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.214
Rwork	0.214
R-free	0.25500
RMSD bond length	0.009
RMSD bond angle	1.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.230
High resolution limit [Å]	2.200	2.200
Rmerge	0.074	0.345 *
Total number of observations	343566 *
Number of reflections	40431 *
<I/σ(I)>	16.4
Completeness [%]	99.6	99.7
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6 *	18 *	1.5 M SODIUM CITRATE AT 290 K, pH 5.60, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM CITRATE
2	1	2	SODIUM CITRATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (%)
2	1	reservoir	sodium citrate	1.1-1.2 (M)