1QSF

STRUCTURE OF A6-TCR BOUND TO HLA-A2 COMPLEXED WITH ALTERED HTLV-1 TAX PEPTIDE Y8A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	1998-01-13
Detector	ADSC
Spacegroup name	C 1 2 1
Unit cell lengths	224.665, 48.460, 93.739
Unit cell angles	90.00, 90.46, 90.00

Refinement procedure

Resolution	25.000 - 2.800
Rwork	0.245
R-free	0.28600
RMSD bond length	0.010 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.063	0.215
Number of reflections	21020
<I/σ(I)>	13.5
Completeness [%]	87.9	87.9
Redundancy	2.2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *	290	seeding with hanging drop vapor diffusion; 50 mM MOPS, pH 7.0, 75 mM MgSO4 and 13% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12 (mg/ml)
2	1	1	HLA-A2/peptide complexes
3	1	1	MES	10 (mM)
4	1	1		50 (mM)
5	1	1	EDTA	0.5 (mM)