1QSC

CRYSTAL STRUCTURE OF THE TRAF DOMAIN OF TRAF2 IN A COMPLEX WITH A PEPTIDE FROM THE CD40 RECEPTOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	110
Detector technology	CCD
Detector	NULL
Spacegroup name	P 21 21 21
Unit cell lengths	87.110, 90.090, 92.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.400
R-factor	0.215
Rwork	0.215
R-free	0.25700
RMSD bond length	0.006
RMSD bond angle	24.700 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.510
High resolution limit [Å]	2.400	2.400
Rmerge	0.048 *
Number of reflections	28075
<I/σ(I)>	9.1
Completeness [%]	98.7	94.6
Redundancy	7.2	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7	20 *	MME-PEG 2000, MAGNESIUM CHLORIDE, BISTRIS PROPANE, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298.00K
1	Vapor diffusion *	7	20 *	MME-PEG 2000, MAGNESIUM CHLORIDE, BISTRIS PROPANE, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 298.00K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEGMME2000	20 (%)
3	1	reservoir	Bis-Tris-propane	0.1 (M)
4	1	reservoir		0.2 (M)