1QSB
THE INTRODUCTION OF STRAIN AND ITS EFFECTS ON THE STRUCTURE AND STABILITY OF T4 LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 60.990, 60.990, 96.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.100 - 1.800 |
| Rwork | 0.160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CYS-FREE VERSION OF T4 LYSOZYME |
| RMSD bond length | 0.019 |
| RMSD bond angle | 14.420 * |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 9.100 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.038 | |
| Number of reflections | 18718 | |
| <I/σ(I)> | 9.698 | 1.76 |
| Completeness [%] | 90.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | phosphate | 2.0 (M) | |
| 3 | 1 | reservoir | 0.25 (M) | ||
| 4 | 1 | reservoir | oxidized beta-mercaptoethanol |
