1QS9

THE INTRODUCTION OF STRAIN AND ITS EFFECTS ON THE STRUCTURE AND STABILITY OF T4 LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 32 2 1
Unit cell lengths	61.062, 61.062, 97.187
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.850
Rwork	0.156
RMSD bond length	0.015
RMSD bond angle	2.400 *
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.200	1.920
High resolution limit [Å]	1.850	1.850
Rmerge	0.054	0.366
Number of reflections	16848
<I/σ(I)>	10.4
Completeness [%]	85.0 *	97
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	POTASSIUM PHOSPHATE, SODIUM PHOSPHATE, BME, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	reservoir	phosphate	2.0 (M)
3	1	reservoir		0.25 (M)
4	1	reservoir	oxidized beta-mercaptoethanol