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CRYSTAL STRUCTURE OF HUMAN A6 TCR COMPLEXED WITH HLA-A2 BOUND TO ALTERED HTLV-1 TAX PEPTIDE P6A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ELLIOTT GX-13
Temperature [K]	110
Detector technology	AREA DETECTOR
Collection date	1997-11-26
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	226.953, 48.757, 95.551
Unit cell angles	90.00, 90.79, 90.00

Refinement procedure

Resolution	25.000 - 2.800
R-factor	0.216 *
Rwork	0.216
R-free	0.27300
RMSD bond length	0.007 *
RMSD bond angle	1.270 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.076	0.260
Number of reflections	25752
<I/σ(I)>	15.1
Completeness [%]	98.2 *	83.2 *
Redundancy	3.1	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *	287	seeding with hanging drop vapor diffusion; 50 mM MOPS pH 7.0, 75 mM MgSO4, 13% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 287K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12 (mg/ml)
2	1	1	MES	10 (mM)
3	1	1		50 (mM)
4	1	1	EDTA	0.5 (mM)
5	1	1	HLA-A2/peptide complexes