1QRH
X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN R145K MUTATION AT 2.7 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | SIEMENS-NICOLET X100 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 118.400, 118.400, 49.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.500 |
| Rwork | 0.161 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.577 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.590 |
| High resolution limit [Å] | 2.440 | 2.440 |
| Rmerge | 0.080 | 0.132 |
| Number of reflections | 8208 | |
| Completeness [%] | 54.0 | 5 |
| Redundancy | 5.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PRECIPITANT: 15% PEG 400, 40 MM BTP, PH 7.1; RESERVOIR: 15% PEG 3350; 40 MM BTP; PH 6.5; DROP:3 UL PROTEIN, 2 UL DNA, 1.5 UL PPT, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PPT | ||
| 2 | 1 | 1 | PEG 400 | ||
| 3 | 1 | 1 | BTP | ||
| 4 | 1 | 2 | PEG 3350 | ||
| 5 | 1 | 2 | BTP |
