1QRB
PLASTICITY AND STERIC STRAIN IN A PARALLEL BETA-HELIX: RATIONAL MUTATIONS IN P22 TAILSPIKE PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Detector technology | AREA DETECTOR |
Collection date | 1998-08-14 |
Detector | MARRESEARCH |
Spacegroup name | P 21 3 |
Unit cell lengths | 120.900, 120.900, 120.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.000 |
R-factor | 0.156 |
Rwork | 0.156 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.640 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.094 | 0.233 |
Total number of observations | 167323 * | |
Number of reflections | 38358 | |
<I/σ(I)> | 7 | |
Completeness [%] | 96.8 | 79.7 |
Redundancy | 4.4 | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 10 | 4 * | Steinbacher, S., (1994) Science, 265, 383. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | ammonium sulfate | 1.0 (M) | |
2 | 1 | reservoir | sodium phosphate | 0.1 (M) | |
3 | 1 | drop | protein | 10 (mg/ml) | |
4 | 1 | drop | HEPES | 10 (mM) | |
5 | 1 | drop | ammonium sulfate | 1.5 (M) | |
6 | 1 | drop | sodium phosphate | 0.1 (M) |