1QR9

INHIBITION OF HIV-1 INFECTIVITY BY THE GP41 CORE: ROLE OF A CONSERVED HYDROPHOBIC CAVITY IN MEMBRANE FUSION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1998-06-20
Detector	RIGAKU RAXIS IV
Spacegroup name	H 3
Unit cell lengths	49.675, 49.675, 60.195
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.600
R-factor	0.202
Rwork	0.202
R-free	0.26100
RMSD bond length	0.005
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.036 *	0.138
Total number of observations	29806 *
Number of reflections	7043
<I/σ(I)>	33.4
Completeness [%]	96.6	69.1
Redundancy	4.2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	SODIUM ACETATE, AMMONIUM SULFATE, GLYCEROL, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	10 (mg/ml)
2	1	reservoir	sodium acetate	0.1 (M)
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	glycerol	20 (%(v/v))