1QR4

TWO FIBRONECTIN TYPE-III DOMAIN SEGMENT FROM CHICKEN TENASCIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	45.240, 57.940, 72.240
Unit cell angles	90.00, 91.40, 90.00

Refinement procedure

Resolution	17.800 - 2.550
R-factor	0.209
Rwork	0.209
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fnf
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.640
High resolution limit [Å]	2.550	2.550
Rmerge	0.074	0.470
Total number of observations	68031 *
Number of reflections	11344
<I/σ(I)>	13	2
Completeness [%]	94.0	75.5 *
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5 *		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	PEG2000	27 (%)
3	1	drop		100 (mM)
4	1	drop		50-100 (mM)