1QQM
D199S MUTANT OF BOVINE 70 KILODALTON HEAT SHOCK PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 143.455, 64.211, 46.488 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.23400 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.020 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.062 | 0.089 |
| Total number of observations | 86567 * | |
| Number of reflections | 30759 | 5255 * |
| <I/σ(I)> | 23 | |
| Completeness [%] | 88.5 | 92.1 |
| Redundancy | 2.8 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 9 | 277 | POLYETHYLENE GLYCOL 8000, POTASSIUM CHLORIDE, pH 9, VAPOR DIFFUSION, HANGING DROP, temperature 4K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG8000 | 20 (%) | |
| 2 | 1 | 1 | 1 (M) | ||
| 3 | 1 | 1 | CHES/KOH | 50 (mM) | |
| 4 | 1 | 2 | ATP | 1 (mM) | |
| 5 | 1 | 2 | 5 (mM) |
