1QQK
THE CRYSTAL STRUCTURE OF FIBROBLAST GROWTH FACTOR 7 (KERATINOCYTE GROWTH FACTOR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 1999-01-01 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 169.100, 169.100, 62.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.100 |
| R-factor | 0.233 * |
| Rwork | 0.233 |
| R-free | 0.32400 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.131 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.210 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.129 | 0.447 |
| Total number of observations | 48247 * | |
| Number of reflections | 8036 | |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 94.2 | 81.6 |
| Redundancy | 6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 * | 298 | SODIUM PHOSPHATE, POTASSIUM PHOSPHATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
| 3 | 1 | reservoir | sodium potassium phosphate | 2.56 (M) | pH5.0 |
| 4 | 1 | reservoir | sodium citrate | 0.1 (M) |
