1QQK

THE CRYSTAL STRUCTURE OF FIBROBLAST GROWTH FACTOR 7 (KERATINOCYTE GROWTH FACTOR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	99
Detector technology	CCD
Collection date	1999-01-01
Detector	ADSC QUANTUM 4
Spacegroup name	I 41 2 2
Unit cell lengths	169.100, 169.100, 62.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.100
R-factor	0.233 *
Rwork	0.233
R-free	0.32400
RMSD bond length	0.015
RMSD bond angle	2.131
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.129	0.447
Total number of observations	48247 *
Number of reflections	8036
<I/σ(I)>	12.1
Completeness [%]	94.2	81.6
Redundancy	6	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7 *	298	SODIUM PHOSPHATE, POTASSIUM PHOSPHATE, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	HEPES	10 (mM)	pH7.0
3	1	reservoir	sodium potassium phosphate	2.56 (M)	pH5.0
4	1	reservoir	sodium citrate	0.1 (M)