1QQF

N-TERMINALLY TRUNCATED C3D,G FRAGMENT OF THE COMPLEMENT SYSTEM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-01-01
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	68.430, 68.430, 117.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.450
R-factor	0.161
R-free	0.21200 *
RMSD bond length	0.010
RMSD bond angle	1.900 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.900	1.620
High resolution limit [Å]	1.450	1.450
Rmerge	0.036	0.285
Number of reflections	50041	13685 *
<I/σ(I)>	8.04
Completeness [%]	99.0	97.2
Redundancy	4.3	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.4 *	15 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	Tris-HCl	0.01 (M)
3	1	reservoir	PEG6000	25-30 (%(w/v))
4	1	reservoir	potassium phosphate	0.05 (M)