1QQE
CRYSTAL STRUCTURE OF THE VESICULAR TRANSPORT PROTEIN SEC17
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-02-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 41 |
| Unit cell lengths | 107.900, 107.900, 46.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.730 - 2.900 |
| R-factor | 0.259 * |
| Rwork | 0.260 |
| R-free | 0.28300 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.054 | 0.472 |
| Number of reflections | 11997 | |
| <I/σ(I)> | 23.6 | |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.9 | 4 * | POLYETHYLENEIMINE, SODIUM CITRATE, SODIUM CHLORIDE, GLYCEROL, DTT, pH 5.9, MICROBATCH, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | polyethyleneimine | 1.5-2. (%) | |
| 5 | 1 | reservoir | glycerol | 3 (%) | |
| 6 | 1 | reservoir | dithiothreitol | 10 (mM) |
