1QQA
PURINE REPRESSOR MUTANT-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 298 |
Detector technology | AREA DETECTOR |
Collection date | 1996-03-06 |
Detector | UCSD MARK III |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 176.230, 95.780, 80.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 3.000 |
R-factor | 0.154 |
RMSD bond length | 0.016 |
RMSD bond angle | 1.900 |
Data reduction software | UCSD-system |
Data scaling software | UCSD-system |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 3.230 | |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.075 * | |
Total number of observations | 56297 * | |
Number of reflections | 13539 | |
<I/σ(I)> | 7.22 | |
Completeness [%] | 100.0 | 84.7 |
Redundancy | 4.2 | 1.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 4000, (NH4)3PO4, (NH4)2SO4, [CO(NH3)6]CL3, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | [CO(NH3)6]CL3 | ||
2 | 1 | 1 | (NH4)3PO4 | ||
3 | 1 | 1 | NA2SO4 | ||
4 | 1 | 1 | PEG 4000 | ||
5 | 1 | 2 | (NH4)3PO4 | ||
6 | 1 | 2 | NA2SO4 | ||
7 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.5 (mM) | |
10 | 1 | reservoir | PEG4000 | 25 (%) | |
11 | 1 | reservoir | ammonium sulphate | 0.4 (M) | |
12 | 1 | reservoir | cobalt hexammine | 50 (mM) | |
13 | 1 | reservoir | ammonium phosphate | 0.1 (M) | |
2 | 1 | drop | dithiothreitol | 1.0 (mM) | |
3 | 1 | drop | sodium potassium phosphate | 160 (mM) | |
4 | 1 | drop | DNA | 0.6 (mM) | |
5 | 1 | drop | sodium cacodylate | 50 (mM) | |
6 | 1 | drop | PEG4000 | 25 (%) | |
7 | 1 | drop | ammonium sulfate | 0.4 (%) | |
8 | 1 | drop | cobalt hexammine | 50 (mM) | |
9 | 1 | drop | ammonium phosphate | 0.1 (M) |