1QQ2

CRYSTAL STRUCTURE OF A MAMMALIAN 2-CYS PEROXIREDOXIN, HBP23.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-C
Temperature [K]	98
Detector technology	IMAGE PLATE
Collection date	1998-10-28
Detector	RIGAKU RAXIS IV
Spacegroup name	P 41 21 2
Unit cell lengths	73.900, 73.900, 211.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.216
Rwork	0.216
R-free	0.26800
RMSD bond length	0.018
RMSD bond angle	26.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (98.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.079	0.335
Number of reflections	262009
<I/σ(I)>	14.3
Completeness [%]	88.9	72.5
Redundancy	8.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6		PEG 6000, POTASSIUM PHOSPHATE, SODIUM CHLORIDE, CHAPS, pH 6.00, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	sodium phosphate	20 (mM)
3	1	drop	CHAPS	1 (mM)
4	1	reservoir		2 (M)
5	1	reservoir	PEG6000	10 (%)