1QPP
CRYSTAL STRUCTURES OF SELF CAPPING PAPD CHAPERONE HOMODIMERS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X31 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-06-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 176.360, 55.340, 46.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.600 |
| Rwork | 0.203 |
| R-free | 0.28600 |
| RMSD bond length | 0.006 * |
| RMSD bond angle | 1.328 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.072 | 0.277 |
| Total number of observations | 53166 * | |
| Number of reflections | 12852 | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 87.7 | 91.1 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 * | 298 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | KMES | 20 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 16-22 (%) | |
| 4 | 1 | reservoir | isopropyl alcohol | 14 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
