1QPH
CRYSTAL STRUCTURE OF THE A-DNA DODECAMER GACCACGTGGTC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | MACSCIENCE |
| Temperature [K] | 278 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-25 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 46.300, 46.300, 72.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.500 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| Structure solution method | RIGID BODY REFINEMENT |
| Starting model (for MR) | NDB ID ADL045 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.000 |
| Data reduction software | AUTOMAR |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 23.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.054 |
| Number of reflections | 1781 |
| <I/σ(I)> | 43.1 |
| Completeness [%] | 97.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | (NH4)2SO4, WATER, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 2 | (NH4)2SO4 |
