1QP9
STRUCTURE OF HAP1-PC7 COMPLEXED TO THE UAS OF CYC7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-09-11 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.900, 90.900, 96.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.800 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| R-free | 0.29400 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.529 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.750 |
| Rmerge | 0.078 * | 0.167 |
| Total number of observations | 27006 * | |
| Number of reflections | 14650 * | |
| <I/σ(I)> | 28.8 | |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 14.77 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 20 * | 11% PEG 400, 10 MM MGSO4, 200 MM KCL, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | MGSO4 | ||
| 3 | 1 | 1 | KCL | ||
| 4 | 1 | 2 | PEG 400 | ||
| 5 | 1 | 2 | MGSO4 | ||
| 6 | 1 | 2 | KCL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.3 (mM) | |
| 2 | 1 | drop | DNA duplex | 0.4 (mM) | |
| 3 | 1 | drop | MES | 25 (mM) | |
| 4 | 1 | drop | PEG400 | 5.5 (%) | |
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | 100 (mM) | ||
| 7 | 1 | reservoir | PEG400 | 11 (%) |
