1QP4

PURINE REPRESSOR-HYPOXANTHINE-PALINDROMIC OPERATOR COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1996-02-14
Detector	UCSD MARK III
Spacegroup name	C 2 2 21
Unit cell lengths	176.480, 95.260, 81.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 3.000
RMSD bond length	0.015
RMSD bond angle	1.800
Data reduction software	UCSD-system
Data scaling software	UCSD-system
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	3.230
High resolution limit [Å]	3.000	3.000
Rmerge	0.080 *
Total number of observations	58105 *
Number of reflections	13547
<I/σ(I)>	9.32
Completeness [%]	99.0	89.6
Redundancy	4.3	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 4000, (NH4)2SO4, [CO(NH3)6]CL3, NA2SO4, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	[CO(NH3)6]CL3
2	1	1	(NH4)2SO4
3	1	1	NA2SO4
4	1	1	PEG 4000
5	1	2	(NH4)2SO4
6	1	2	NA2SO4
7	1	2	PEG 4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (mM)
10	1	reservoir	PEG4000	25 (%)
11	1	reservoir	ammonium sulphate	0.4 (M)
12	1	reservoir	cobalt hexammine	50 (mM)
13	1	reservoir	ammonium phosphate	0.1 (M)
2	1	drop	dithiothreitol	1.0 (mM)
3	1	drop	sodium potassium phosphate	160 (mM)
4	1	drop	DNA	0.6 (mM)
5	1	drop	sodium cacodylate	50 (mM)
6	1	drop	PEG4000	25 (%)
7	1	drop	ammonium sulfate	0.4 (%)
8	1	drop	cobalt hexammine	50 (mM)
9	1	drop	ammonium phosphate	0.1 (M)