1QP1

KAPPA VARIABLE LIGHT CHAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1992-08-11
Detector	RIGAKU RAXIS II
Spacegroup name	C 2 2 21
Unit cell lengths	143.990, 82.560, 77.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.060
R-factor	0.188
Rwork	0.153
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	THE BENCE-JONES PROTEINS ROY: COLMAN SCHRAMM GURS J. MOL. BIOL. 116 73 1977 AND REI: EPP COLMAN FEHLHAMMER BODE SCHIFFER HUBER AND PALM EUR. J. BIOCHEM. 45 513 1974
RMSD bond length	0.015
RMSD bond angle	0.000
Phasing software	X-PLOR
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.180
High resolution limit [Å]	2.060	2.060
Rmerge	0.120	0.200
Number of reflections	22485
<I/σ(I)>	24	1.92
Completeness [%]	68.9	50.2 *
Redundancy	5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4	300	AMMONIUM SULFATE, CITRATE BUFFER, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 300K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	ammonium sulfate	60 (%)
3	1	1	citrate	200 (mM)