1QOZ
Catalytic core domain of acetyl xylan esterase from Trichoderma reesei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.300, 61.000, 40.000 |
| Unit cell angles | 107.70, 113.50, 98.80 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.186 |
| Rwork | 0.186 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bs9 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.650 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.057 * | 0.247 * |
| Total number of observations | 55958 * | |
| Number of reflections | 27058 | |
| <I/σ(I)> | 14.9 | 2.38 |
| Completeness [%] | 87.0 | 47 * |
| Redundancy | 2.07 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.2 | 1.1 M POTASSIUM/SODIUM TARTRATE, 0.1 M TES, 9 MM TRITON-X, PH 8.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Triton X-100 solution | 2 (mM) | |
| 2 | 1 | drop | protein | 5 (mg/ml) | pH5.0 |
| 3 | 1 | drop | sodium acetate | ||
| 4 | 1 | drop | potassium/sodium tartrate | 0.28 (M) | |
| 5 | 1 | drop | TES | 0.025 (M) | |
| 6 | 1 | reservoir | potassium/sodium tartrate | 1.1 (M) | |
| 7 | 1 | reservoir | TES | 0.1 (M) | pH8.2 |
