1QOY
E.coli Hemolysin E (HlyE, ClyA, SheA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-05-29 |
| Detector | ADSC QUANTUM 4 CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 84.470, 84.470, 142.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 17.800 - 2.000 |
| Rwork | 0.198 |
| R-free | 0.25200 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 15.100 * |
| Data reduction software | MOSFLM |
| Data scaling software | Agrovata |
| Phasing software | CCP4 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.800 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.036 | 0.056 |
| Total number of observations | 78696 * | |
| Number of reflections | 31955 | |
| <I/σ(I)> | 12 | 7.6 |
| Completeness [%] | 89.3 | 72 |
| Redundancy | 2.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 25 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | 2.5 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 1.1 (M) | |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | MES | 100 (mM) |
