1QOX
Beta-glucosidase from Bacillus circulans sp. alkalophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 116.400, 122.300, 163.200 |
| Unit cell angles | 89.30, 74.30, 68.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.255 |
| Rwork | 0.255 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bga |
| RMSD bond length | 0.004 |
| RMSD bond angle | 22.650 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.780 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.076 * | 0.322 * |
| Total number of observations | 303163 * | |
| Number of reflections | 186165 | |
| <I/σ(I)> | 8.7 | 1.7 |
| Completeness [%] | 83.7 | 66 |
| Redundancy | 1.63 | 1.47 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 13 % PEG8000, 0.3 M SODIUM FORMATE, 0.1 M SODIUM ACETATE, PH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris(tris(hydroxymethyl)aminomethane) | ||
| 3 | 1 | reservoir | PEG8000 | 13 (%) | |
| 4 | 1 | reservoir | sodium formate | 0.3 (M) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
