1QOX

Beta-glucosidase from Bacillus circulans sp. alkalophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-08-15
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 1
Unit cell lengths	116.400, 122.300, 163.200
Unit cell angles	89.30, 74.30, 68.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.255
Rwork	0.255
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bga
RMSD bond length	0.004
RMSD bond angle	22.650 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000	2.780
High resolution limit [Å]	2.690	2.690
Rmerge	0.076 *	0.322 *
Total number of observations	303163 *
Number of reflections	186165
<I/σ(I)>	8.7	1.7
Completeness [%]	83.7	66
Redundancy	1.63	1.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		13 % PEG8000, 0.3 M SODIUM FORMATE, 0.1 M SODIUM ACETATE, PH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris(tris(hydroxymethyl)aminomethane)
3	1	reservoir	PEG8000	13 (%)
4	1	reservoir	sodium formate	0.3 (M)
5	1	reservoir	sodium acetate	0.1 (M)