1QOU
CEN (Centroradialis) protein from Antirrhinum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.890, 77.650, 117.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.900 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bd9 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.034 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.057 | 0.226 |
| Number of reflections | 28860 | |
| <I/σ(I)> | 22.4 | 4.6 |
| Completeness [%] | 94.9 | 91.4 |
| Redundancy | 4.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 * | 1:1 MIX OF 13MG/ML CEN SOLUTION (10MM HEPES, 50MM NACL. PH 7) WITH 1.8 - 2.1 M NACL BUFFERED WITH 100MM SODIUM ACETATE AT PH 5.4 - 5.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | HEPES | 10 (mM) | |
| 2 | 1 | 1 | 50 (mM) | ||
| 3 | 1 | 1 | protein | 13 (mg/ml) | |
| 4 | 1 | 2 | 1.8-2.1 (M) | ||
| 5 | 1 | 2 | sodium acetate | 100 (mM) |
