1QOM
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER (DELTA 65) WITH SWAPPED N-TERMINAL HOOK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 212.980, 212.980, 114.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.236 |
| Rwork | 0.236 |
| R-free | 0.30600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.059 * | 0.362 * |
| Total number of observations | 153562 * | |
| Number of reflections | 39200 | 4290 * |
| <I/σ(I)> | 17 | 2.9 |
| Completeness [%] | 91.7 | 92.3 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.6 * | 4 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 2 | 1 | drop | Hepps | 40 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | H4B | 2-4 (mM) | |
| 6 | 1 | reservoir | beta-octyl-glucoside | 50 (mM) | |
| 7 | 1 | reservoir | 18-21 (%) | or ammonium sulfate | |
| 8 | 1 | reservoir | S-ethyl-thiourea | 4 (mM) | or 4mM Fe(II)Cl2 |
