1QOK
MFE-23 AN ANTI-CARCINOEMBRYONIC ANTIGEN SINGLE-CHAIN FV ANTIBODY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-15 |
| Detector | R-AXIS II |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.700, 61.700, 128.000 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.205 |
| Rwork | 0.205 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fbj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.082 | 0.189 |
| Number of reflections | 11539 | 1613 * |
| <I/σ(I)> | 4.4 | 3.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 5.3 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 18 * | CRYSTALS WERE GROWN BY THE HANGING-DROP VAPOUR DIFFUSION METHOD AT 18 DEGREES (CELSIUS). PROTEIN SOLUTION (2 MG/ML) WAS MIXED 1:1 WITH 100 MM TRIS-HCL (PH6.5) CONTAINING 45% SATURATED AMMONIUM SULPHATE. A 10 UL DROPLET OF THIS MIXTURE WAS EQULIBRATED AGAINST 0.5 ML 100 MM TRIS-HCL (PH6.5) IN 45% AMMONIUM SULPHATE., pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | ammonium sulfate | 22.5 (%sat) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 45 (%sat) |
