1QOK

MFE-23 AN ANTI-CARCINOEMBRYONIC ANTIGEN SINGLE-CHAIN FV ANTIBODY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1994-03-15
Detector	R-AXIS II
Spacegroup name	P 32 2 1
Unit cell lengths	61.700, 61.700, 128.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.205
Rwork	0.205
R-free	0.26700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fbj
RMSD bond length	0.010
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.082	0.189
Number of reflections	11539	1613 *
<I/σ(I)>	4.4	3.2
Completeness [%]	99.6	100
Redundancy	5.3	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	18 *	CRYSTALS WERE GROWN BY THE HANGING-DROP VAPOUR DIFFUSION METHOD AT 18 DEGREES (CELSIUS). PROTEIN SOLUTION (2 MG/ML) WAS MIXED 1:1 WITH 100 MM TRIS-HCL (PH6.5) CONTAINING 45% SATURATED AMMONIUM SULPHATE. A 10 UL DROPLET OF THIS MIXTURE WAS EQULIBRATED AGAINST 0.5 ML 100 MM TRIS-HCL (PH6.5) IN 45% AMMONIUM SULPHATE., pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	1 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	ammonium sulfate	22.5 (%sat)
4	1	reservoir	Tris-HCl	100 (mM)
5	1	reservoir	ammonium sulfate	45 (%sat)