1QOJ
Crystal Structure of E.coli UvrB C-terminal domain, and a model for UvrB-UvrC interaction.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-05-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8856, 0.9788, 0.9789 |
| Spacegroup name | P 62 |
| Unit cell lengths | 84.810, 84.810, 53.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.3266 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.022 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.102 | 0.511 |
| Total number of observations | 65208 * | |
| Number of reflections | 4450 | |
| <I/σ(I)> | 2.75 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.86 | 3.71 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 5 * | BY VAPOUR DIFFUSION 3UL PROTEIN SOLUTION: 2MG/ML UVRB DOMAIN, 20MM TRIS-CL PH7.0, 150MM NACL 0.1% NAN3 + 3UL WELL SOLUTION 0.5ML WELL SOLUTION: 35% SAT. AMMONIUM SULPHATE 100MM TRIS-CL PH8.8, 0.1% NAN3, pH 8.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 150 (mM) | ||
| 4 | 1 | drop | 0.1 (%) | ||
| 5 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | 0.1 (%) |
